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Checking the level of nicotine in your e-liquids

Nicotine is a natural alkaloid present in certain plants such as tomato, aubergine or potato. It is an agent of protection against parasites. Tobacco is the plant that contains it most (around a few %). The level of nicotine present in these plants depends on the tobacco variety and also the farming techniques used.

Detection method


Volume required


Analysis time

3 working days

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What are the effects of nicotine on humans?

Taken in low doses, nicotine has a stimulating effect with humans. It causes and increases of arterial pressure and heart beat as well as the release of adrenaline. However, nicotine is considered as addictive because it creates strong dependency when it is assimilated by the human body. The number of nicotinic receptors increases with nicotine absorption. The more it is consumed, the more receptors there are, and the more the body needs it.

In pure state, it is a lethal substance.

In vaping products, this is used as a smoking cessation tool, the concentration of which is decisive. In an e-liquid, too high a level of nicotine can cause an overdose with side effects such as: headache, vomiting, nausea or illnesses in extreme cases. Conversely, too low a level of nicotine can cause a feeling of shortage and make smoking cessation using a personal vaporiser ineffective.

Why analyse the level of nicotine in e-liquids?

Vaping products are considered as chemical products. Therefore, they have to comply with the CLP information requirements (Classification for Labelling and Packaging). This is a European regulation which aims to prevent chemical risks. Thus, the presence of nicotine in an e-liquid must be given with its exact level on its label. The aim is to provide precise information to the consumer.

Moreover, since 2016 the manufacturers of e-liquids must declare on a European notification platform the composition of all the products containing nicotine (e-liquid and emission), in application of the TPD, the European directive on tobacco products. For this, all the components of an e-liquid having a concentration greater than 0.1% must be known and quantified. It is necessary then to know the precise concentration of nicotine present in the finished product in order to make this declaration.

Support from ingésciences

Our OpenSciences laboratory analyses, for e-liquid manufacturers, wholesalers and marketing managers, the level of nicotine in their products. Our scientific team has developed suitable methods for the analysis of the products in liquid state and in emission. After analysis of the samples, we produce a full report on request. Finally, for our customers we check the compliance of the labelling of their product.

Do you require an analysis?

Check the level of nicotine in your products?